Analysis Note

Positive ControlPRC/PRF/5 cells, A431 cells, Human small intestine tissue, human colon tissue

Negative Control293T cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Paraffin Sections (3-5 µg/ml)

General description

Purified mouse monoclonal antibody. Recognizes the ~17 kDa CLDN1 protein.

This Anti-CLDN1 Mouse mAb (1C5-D9) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of CLDN1.

Recognizes the ~17 kDa CLDN1 protein in PRC/PRF/5 and A431 cells and human small intestine and colon tissue.

Immunogen

Full-length, recombinant, human CLDN1 (aa 1-212), expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Guevin C, et al. 2009. Antiviral Res.84, 14.Sainz, B., Jr, et al. 2009. Virol. J.6, 103.Stamataki Z, et al. 2009. Blood113 585.Farquhar MJ, et al. 2008. J. Virol.82 8797.

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)